catalog number :
MBS908239
products type :
ELISA Kit
products full name :
Mouse Interleukin-22 receptor subunit alpha-2, IL22RA2 ELISA Kit
products short name :
interleukin 22 receptor, alpha 2
products name syn :
Mouse Interleukin-22 receptor subunit alpha-2 (IL22RA2) ELISA kit; CRF2-10; CRF2-S1; CRF2X; IL-22BP; MGC150509; MGC150510; class II cytokine receptor; interleukin 22-binding protein; interleukin 22 receptor; alpha 2
other names :
interleukin-22 receptor subunit alpha-2; Interleukin-22 receptor subunit alpha-2; interleukin-22 receptor subunit alpha-2; IL22BP; IL-22RA2; zcytoR16; IL-22R-alpha-2; IL-22 receptor subunit alpha-2; interleukin-22-binding protein; cytokine receptor family II soluble 1; cytokine receptor family type 2, soluble 1; interleukin 22 receptor, alpha 2; Cytokine receptor family type 2, soluble 1; CRF2-S1; Interleukin-22-binding protein; IL-22BP; IL22BP; ZcytoR16
products gene name :
IL22RA2
other gene names :
Il22ra2; Il22ra2; CRF2X; CRF2-10; CRF2-s1; Il-22bp; IL-22 receptor subunit alpha-2; IL-22R-alpha-2; IL-22RA2; CRF2-S1; IL-22BP; IL22BP
uniprot entry name :
I22R2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse IL22RA2. No significant cross-reactivity or interference between mouse IL22RA2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of mouse IL22RA2 is typically less than 11.72 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL22RA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL22RA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL22RA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL22RA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_839989.2
ncbi gb acc num :
NM_178258.5
ncbi mol weight :
26,613 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)
uniprot summary :
IL22RA2: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses. Belongs to the type II cytokine receptor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region; plasma membrane; integral to membrane; cytosol. Molecular Function: protein binding; interleukin-22 binding; interleukin-22 receptor activity. Biological Process: negative regulation of inflammatory response; regulation of tyrosine phosphorylation of Stat3 protein; cytokine and chemokine mediated signaling pathway
size4 :
10x96-Strip-Wells