catalog number :
MBS908180
products type :
ELISA Kit
products full name :
Rat Carboxypeptidase B, CPB1 ELISA Kit
products short name :
carboxypeptidase B1 (tissue)
products name syn :
Rat Carboxypeptidase B (CPB1) ELISA kit; CPB; DKFZp779K1333; PASP; PCPB; pancreas-specific protein; pancreatic carboxypeptidase B1; procarboxypeptidase B; carboxypeptidase B1 (tissue)
other names :
carboxypeptidase B; Carboxypeptidase B; carboxypeptidase B; pancreatic carboxypeptidase B1; carboxypeptidase B1 (tissue)
products gene name :
CPB1
other gene names :
Cpb1; Cpb1; Cpb; Cpb
uniprot entry name :
CBPB1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CPB1. No significant cross-reactivity or interference between rat CPB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 ng/ml-800 ng/ml. Sensitivity: Typically less than 3.12 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036665.1
ncbi gb acc num :
NM_012533.1
ncbi mol weight :
47,515 Da
ncbi pathways :
Pancreatic Secretion Pathway (169303); Pancreatic Secretion Pathway (169295); Protein Digestion And Absorption Pathway (172816); Protein Digestion And Absorption Pathway (171868)
ncbi summary :
putative carboxypeptidase B protein [RGD, Feb 2006]
uniprot summary :
CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]. Protein type: Protease; Secreted; EC 3.4.17.2; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity. Biological Process: proteolysis
size4 :
10x96-Strip-Wells