ELISA/assay

Dog Cystic fibrosis transmembrane conductance regulator, CFTR ELISA Kit

MBS907966

48-Strip-Wells

565 USD

ELISA Kit

Dog Cystic fibrosis transmembrane conductance regulator, CFTR ELISA Kit

cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)

Dog Cystic fibrosis transmembrane conductance regulator (CFTR) ELISA kit; tcag7.78; ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; dJ760C5.1; ATP-binding cassette sub-family C; member 7; cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C; member 7)

cystic fibrosis transmembrane conductance regulator; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel

CFTR

CFTR; CFTR; ABCC7; CFTR

CFTR_CANFA

Dog

1483

This assay has high sensitivity and excellent specificity for detection of Dog CFTR. No significant cross-reactivity or interference between Dog CFTR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.69 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Ion transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CFTR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CFTR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFTR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFTR bound in the initial step. The color development is stopped and the intensity of the color is measured.

55742782

NP_001007144.1

NM_001007143.1

Q5U820

168,491 Da

ABC Transporters Pathway (84085); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (580373); Bile Secretion Pathway (193153); Bile Secretion Pathway (193095); Gastric Acid Secretion Pathway (154414); Gastric Acid Secretion Pathway (154383); Pancreatic Secretion Pathway (169310); Pancreatic Secretion Pathway (169295); Transmembrane Transport Of Small Molecules Pathway (580372)

Function: Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation . Catalytic activity: ATP + H2O = ADP + phosphate.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150