Human Acetyl-CoA carboxylase 1, ACACA ELISA Kit | MyBioSource MBS907630 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Acetyl-CoA carboxylase 1, ACACA ELISA Kit

catalog :

MBS907630

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS907630

products type :

ELISA Kit

products full name :

Human Acetyl-CoA carboxylase 1, ACACA ELISA Kit

products short name :

[acetyl-Coenzyme A carboxylase alpha]

products name syn :

[Human Acetyl-CoA carboxylase 1 (ACACA) ELISA kit; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha]

other names :

[acetyl-CoA carboxylase 1 isoform 1; Acetyl-CoA carboxylase 1; acetyl-CoA carboxylase 1; ACC-alpha; acetyl-Coenzyme A carboxylase alpha; acetyl-CoA carboxylase alpha; ACC-alphaIncluding the following 1 domains:Biotin carboxylase (EC:6.3.4.14)]

products gene name :

[ACACA]

other gene names :

[ACACA; ACACA; ACC; ACAC; ACC1; ACCA; ACACAD; ACAC; ACC1; ACCA; ACC1]

uniprot entry name :

ACACA_HUMAN

reactivity :

Human

sequence length :

2383

specificity :

This assay has high sensitivity and excellent specificity for detection of human ABL1. No significant cross-reactivity or interference between human ABL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: 6.25 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38679960

ncbi acc num :

NP_942131.1

ncbi gb acc num :

NM_198834.2

uniprot acc num :

Q13085

ncbi mol weight :

265,554 Da

ncbi pathways :

AMPK Signaling Pathway (198868); ChREBP Activates Metabolic Gene Expression Pathway (106104); Fatty Acid Biosynthesis Pathway (198873); Fatty Acyl-CoA Biosynthesis Pathway (106115); Fatty Acid Biosynthesis Pathway (82933); Fatty Acid Biosynthesis Pathway (294); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Integration Of Energy Metabolism Pathway (106096)

ncbi summary :

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. Protein type: Lipid Metabolism - fatty acid biosynthesis; Ligase; Carbohydrate Metabolism - propanoate; EC 6.4.1.2; Carbohydrate Metabolism - pyruvate; EC 6.3.4.14. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton. Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding. Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; positive regulation of cellular metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; cellular lipid metabolic process; carnitine shuttle; biotin metabolic process; protein homotetramerization; multicellular organismal protein metabolic process; tissue homeostasis; energy reserve metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process. Disease: Acetyl-coa Carboxylase Deficiency

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage