ELISA/assay

Rat Platelet glycoprotein 4, CD36 ELISA Kit

MBS907629

48-Strip-Wells

510 USD

ELISA Kit

Rat Platelet glycoprotein 4, CD36 ELISA Kit

CD36 molecule (thrombospondin receptor)

Rat Platelet glycoprotein 4 (CD36) ELISA kit; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3; CD36 antigen; CD36 antigen (collagen type I receptor; thrombospondin receptor) ; OTTHUMP00000207872; OTTHUMP00000207874; PAS-4 protein; cluster determinant 36; f; CD36 molecule (thrombospondin receptor)

Platelet glycoprotein 4; Platelet glycoprotein 4; platelet glycoprotein 4; GPIV; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV; adipocyte membrane protein; fatty acid transport protein; collagen type I receptor thrombospondin receptor; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); Adipocyte membrane protein; Fatty acid translocase; Fatty acid transport protein; Glycoprotein IIIb; GPIIIB; PAS IV; PAS-4; Platelet glycoprotein IV; GPIV; CD_antigen: CD36

CD36

Cd36; Cd36; Fat; Fat; GPIIIB; GPIV

CD36_RAT

Rat

472

This assay has high sensitivity and excellent specificity for detection of rat CD36. No significant cross-reactivity or interference between rat CD36 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD36 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound in the initial step. The color development is stopped and the intensity of the color is measured.

146345388

Q07969.3

Q07969

52,731 Da

Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Fat Digestion And Absorption Pathway (194376); Fat Digestion And Absorption Pathway (194324); Hematopoietic Cell Lineage Pathway (83470); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (573899); Malaria Pathway (152662)

fatty acid translocase; involved in long-chain fatty acid (LCFA) transport; important in fatty acid metabolism and insulin function [RGD, Feb 2006]

CD36: Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass. Cellular Component: Golgi apparatus; extracellular space; cell surface; protein complex; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; caveola; lipid raft; membrane; apical part of cell; plasma membrane; intracellular; sarcolemma; external side of plasma membrane. Molecular Function: low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding; low-density lipoprotein binding; high-density lipoprotein binding; lipid binding. Biological Process: positive regulation of blood coagulation; cGMP-mediated signaling; positive regulation of interleukin-12 production; phagocytosis, recognition; negative regulation of transcription from RNA polymerase II promoter; pattern recognition receptor signaling pathway; signal transduction; fatty acid metabolic process; negative regulation of transcription factor import into nucleus; response to estradiol stimulus; low density lipoprotein mediated signaling; sequestering of lipid; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of cell-matrix adhesion; cell adhesion; response to nutrient; long-chain fatty acid transport; response to drug; receptor-mediated endocytosis; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of systemic arterial blood pressure; positive regulation of interleukin-6 production; long-chain fatty acid metabolic process; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; negative regulation of angiogenesis; triacylglycerol metabolic process; gut development; cellular response to insulin stimulus; response to mechanical stimulus; lipoprotein transport; fatty acid oxidation; response to activity; positive regulation of phagocytosis, engulfment; plasma membrane long-chain fatty acid transport; nitric oxide mediated signal transduction; fatty acid transport; apoptotic cell clearance; positive regulation of cytokine secretion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800