catalog number :
MBS907598
products type :
ELISA Kit
products full name :
Dog Carbonic anhydrase 6, CA6 ELISA Kit
products short name :
carbonic anhydrase VI
products name syn :
Dog Carbonic anhydrase 6 (CA6) ELISA kit; RP3-477M7.7; CA-VI; GUSTIN; MGC21256; carbonate dehydratase VI; salivary carbonic anhydrase; secreted carbonic anhydrase; carbonic anhydrase VI
other names :
carbonic anhydrase 6; Carbonic anhydrase 6; carbonic anhydrase 6; CA-VI; carbonate dehydratase VI; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI
other gene names :
CA6; CA6; CA-VI
uniprot entry name :
CAH6_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog CA6. No significant cross-reactivity or interference between dog CA6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 39 pg/ml-2500 pg/ml. Sensitivity: The minimum detectable dose of dog CA6 is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001002999.1
ncbi gb acc num :
NM_001002999.1
ncbi mol weight :
36,705 Da
uniprot summary :
Function: Reversible hydration of carbon dioxide. Its role in saliva is unknown . Catalytic activity: H2CO3 = CO2 + H2O. Cofactor: Zinc
size4 :
10x96-Strip-Wells