This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Histone H2A.x, H2AFX ELISA Kit
catalog :
MBS907486
quantity :
96 Strip Wells
price :
760 USD
product information
catalog number :
MBS907486
products type :
ELISA Kit
products full name :
Mouse Histone H2A.x, H2AFX ELISA Kit
products short name :
H2A histone family, member X
products name syn :
Mouse Histone H2A.x (H2AFX) ELISA kit; H2A.X; H2A/X; H2AX; H2AX histone; H2A histone family; member X
other names :
histone H2AX; Histone H2AX; histone H2AX; H2a/x; histone H2A.x; histone 5 protein 2ax; H2A histone family, member X; Histone H2A.X
products gene name :
H2AFX
other gene names :
H2afx; H2afx; H2ax; H2A.X; AW228881; Hist5-2ax; gammaH2ax; H2a.x; H2ax; Hist5-2ax; H2a/x
uniprot entry name :
H2AX_MOUSE
reactivity :
Mouse
sequence length :
143
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse H2AFX. No significant cross-reactivity or interference between mouse H2AFX and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of mouse histone H2A.x (H2AFX) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 15.6 pg/ml-1000 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of mouse H2AFX is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for H2AFX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any H2AFX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H2AFX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H2AFX bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
7106331
ncbi acc num :
NP_034566.1
ncbi gb acc num :
NM_010436.2
uniprot acc num :
P27661
ncbi mol weight :
15,143 Da
ncbi pathways :
ATM Mediated Phosphorylation Of Repair Proteins Pathway 640618!!ATM Mediated Response To DNA Double-strand Break Pathway 640617!!Alcoholism Pathway 585577!!Alcoholism Pathway 587116!!Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway 640621!!Cell Cycle Pathway 640475!!Chromosome Maintenance Pathway 640567!!DNA Repair Pathway 640590!!Double-Strand Break Repair Pathway 640614!!Homologous Recombination Repair Pathway 640615
uniprot summary :
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.15 PublicationsManual assertion based on experiment in:Ref.8
size :
96 Strip Wells
price :
760 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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