catalog number :
MBS907437
products type :
ELISA Kit
products full name :
Human Plasminogen activator inhibitor 2, SERPINB2 ELISA Kit
products short name :
serpin peptidase inhibitor, clade B (ovalbumin) , member 2
products name syn :
Human Plasminogen activator inhibitor 2 (SERPINB2) ELISA kit; HsT1201; PAI; PAI-2; PAI2; PLANH2; plasminogen activator inhibitor 2; plasminogen activator inhibitor; type II (arginine-serpin) ; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member; serpin peptidase inhibitor; clade B (ovalbumin); member 2
other names :
plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2; plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
products gene name :
SERPINB2
other gene names :
SERPINB2; SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201; PAI2; PLANH2; PAI-2
uniprot entry name :
PAI2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human SERPINB2. No significant cross-reactivity or interference between human SERPINB2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human SERPINB2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001137290.1
ncbi gb acc num :
NM_001143818.1
ncbi mol weight :
46,596 Da
ncbi pathways :
Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Blood Clotting Cascade Pathway (198840); Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Senescence And Autophagy Pathway (198780)
uniprot summary :
SERPINB2: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Belongs to the serpin family. Ov-serpin subfamily. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: fibrinolysis; blood coagulation; negative regulation of apoptosis
size4 :
10x96-Strip-Wells