ELISA/assay

Human Peroxidasin homolog, PXDN ELISA Kit

MBS907356

96 Strip Wells

760 USD

ELISA Kit

Human Peroxidasin homolog, PXDN ELISA Kit

peroxidasin homolog (Drosophila)

Human Peroxidasin homolog (PXDN) ELISA kit; D2S448; D2S448E; KIAA0230; MG50; PRG2; PXN; VPO; p53-responsive gene 2; peroxidasin; peroxidasin homolog (Drosophila)

peroxidasin homolog; Peroxidasin homolog; peroxidasin homolog; vascular peroxidase 1; p53-responsive gene 2 protein; melanoma-associated antigen MG50; peroxidasin homolog (Drosophila); Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein

PXDN

PXDN; PXDN; PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E; KIAA0230; MG50; PRG2; VPO; VPO1

PXDN_HUMAN

Human

1479

This assay has high sensitivity and excellent specificity for detection of Human PXDN. No significant cross-reactivity or interference between Human PXDN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.188 ng/ml-12 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.04 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Oxidative Stress. Protein Biological Process 3: Hydrogen peroxide

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PXDN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PXDN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDN bound in the initial step. The color development is stopped and the intensity of the color is measured.

109150416

NP_036425.1

NM_012293.2

Q92626

165,275 Da

This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]

Function: Displays low peroxidase activity and is likely to participate in H2O2 metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation.2 PublicationsManual assertion based on experiment in:Ref.3. Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.2 PublicationsManual assertion based on experiment in:Ref.3. Cofactor: Binds 1 calcium ion per subunit 1 PublicationManual assertion based on experiment in:Ref.3

96 Strip Wells

760 USD