Human Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1 ELISA Kit | MyBioSource MBS907317 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1 ELISA Kit

catalog :

MBS907317

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS907317

products type :

ELISA Kit

products full name :

Human Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1 ELISA Kit

products short name :

ectonucleoside triphosphate diphosphohydrolase 1

products name syn :

Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) ELISA kit; ATPDase; CD39; DKFZp686D194; DKFZp686I093; FLJ40921; FLJ40959; NTPDase-1; CD39 antigen; NTPDase 1; ecto-ATP diphosphohydrolase; ecto-apyrase; lymphoid cell activation antigen; ectonucleoside triphosphate diphosphohydrolase 1

other names :

ectonucleoside triphosphate diphosphohydrolase 1 isoform 2; Ectonucleoside triphosphate diphosphohydrolase 1; ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; CD39 antigen; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen; ectonucleoside triphosphate diphosphohydrolase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; CD_antigen: CD39

products gene name :

ENTPD1

other gene names :

ENTPD1; ENTPD1; CD39; SPG64; ATPDase; NTPDase-1; CD39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1

uniprot entry name :

ENTP1_HUMAN

reactivity :

Human

sequence length :

517

specificity :

This assay has high sensitivity and excellent specificity for detection of human NETPD1. No significant cross-reactivity or interference between human NETPD1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human NETPD1 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENTPD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENTPD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENTPD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENTPD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

147905700

ncbi acc num :

NP_001091645.1

ncbi gb acc num :

NM_001098175.1

uniprot acc num :

P49961

ncbi mol weight :

57,965 Da

ncbi pathways :

Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

uniprot summary :

ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Hydrolase; EC 3.6.1.5; Extracellular matrix. Chromosomal Location of Human Ortholog: 10q24. Cellular Component: membrane; integral to plasma membrane; plasma membrane; basal lamina. Molecular Function: protein binding; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding. Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; cell adhesion; purine ribonucleoside diphosphate catabolic process; blood coagulation. Disease: Spastic Paraplegia 64, Autosomal Recessive

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage