catalog number :
MBS907252
products type :
ELISA Kit
products full name :
Human B-cell differentiation antigen CD72, CD72 ELISA Kit
products short name :
CD72 molecule
products name syn :
Human B-cell differentiation antigen CD72 (CD72) ELISA kit; RP11-331F9.9; CD72b; LYB2; CD72 antigen; OTTHUMP00000021342; CD72 molecule
other names :
B-cell differentiation antigen CD72; B-cell differentiation antigen CD72; B-cell differentiation antigen CD72; lyb-2; CD72 antigen; CD72 molecule; Lyb-2; CD_antigen: CD72
products gene name :
CD72
other gene names :
CD72; CD72; LYB2; CD72b
uniprot entry name :
CD72_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CD72. No significant cross-reactivity or interference between human CD72 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.9 ng/ml-60 ng/ml. Sensitivity: The minimum detectable dose of human CD72 is typically less than 0.225 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD72 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001773.1
ncbi gb acc num :
NM_001782.2
ncbi mol weight :
40,220 Da
ncbi pathways :
Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Developmental Biology Pathway (477129); Sema4D In Semaphorin Signaling Pathway (119520); Semaphorin Interactions Pathway (119519)
uniprot summary :
CD72: Plays a role in B-cell proliferation and differentiation. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding; receptor binding. Biological Process: axon guidance; signal transduction; cell adhesion
size4 :
10x96-Strip-Wells