ELISA/assay

Human Laminin subunit alpha-4, LAMA4 ELISA Kit

MBS907209

96 Strip Wells

760 USD

ELISA Kit

Human Laminin subunit alpha-4, LAMA4 ELISA Kit

laminin, alpha 4

Human Laminin subunit alpha-4 (LAMA4) ELISA kit; RP1-142L7.4; DKFZp686D23145; LAMA3; LAMA4*-1; laminin alpha 4 chain; laminin; alpha 4

laminin subunit alpha-4 isoform 1; Laminin subunit alpha-4; laminin subunit alpha-4; laminin alpha 4 chain; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha

LAMA4

LAMA4; LAMA4; LAMA3; CMD1JJ; LAMA4*-1

LAMA4_HUMAN

Human

1823

This assay has high sensitivity and excellent specificity for detection of human LAMA4. No significant cross-reactivity or interference between human LAMA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human laminin subunit alpha-4 (LAMA4) concentrations in serum, plasma, tissue homogenates. Detection Range: 23.5 pg/ml-1500 pg/ml.

Sensitivity: The minimum detectable dose of human LAMA4 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

380503843

NP_001098676.2

NM_001105206.2

Q16363

202,524 Da

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Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

96 Strip Wells

760 USD