catalog number :
MBS907149
products type :
ELISA Kit
products full name :
Human Matrix-remodeling-associated protein 8, MXRA8 ELISA Kit
products short name :
matrix-remodelling associated 8
products name syn :
Human Matrix-remodeling-associated protein 8 (MXRA8) ELISA kit; DKFZp586E2023; MGC3047; limitrin; matrix-remodelling associated 8
other names :
matrix-remodeling-associated protein 8 isoform 2; Matrix-remodeling-associated protein 8; matrix-remodeling-associated protein 8; limitrin; matrix-remodelling associated 8; Limitrin
products gene name :
MXRA8
other gene names :
MXRA8; MXRA8
uniprot entry name :
MXRA8_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MXRA8. No significant cross-reactivity or interference between human MXRA8 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human MXRA8 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MXRA8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MXRA8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MXRA8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MXRA8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001269511.1
ncbi gb acc num :
NM_001282582.1
ncbi mol weight :
49,132 Da
uniprot summary :
MXRA8: May play a role in the maturation and maintenance of blood-brain barrier. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 1p36.33. Cellular Component: cell surface; integral to membrane
size4 :
10x96-Strip-Wells