catalog number :
MBS907060
products type :
ELISA Kit
products full name :
Human Procollagen C-endopeptidase enhancer 2, PCOLCE2 ELISA Kit
products short name :
procollagen C-endopeptidase enhancer 2
products name syn :
Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA kit; PCPE2; ; procollagen C-endopeptidase enhancer 2
other names :
procollagen C-endopeptidase enhancer 2; Procollagen C-endopeptidase enhancer 2; procollagen C-endopeptidase enhancer 2; procollagen C-proteinase enhancer 2; procollagen COOH-terminal proteinase enhancer 2; procollagen C-endopeptidase enhancer 2; Procollagen COOH-terminal proteinase enhancer 2; PCPE-2; Procollagen C-proteinase enhancer 2
products gene name :
PCOLCE2
other gene names :
PCOLCE2; PCOLCE2; PCPE2; PCPE2; UNQ250/PRO287; PCPE-2; Procollagen C-proteinase enhancer 2
uniprot entry name :
PCOC2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human PCOLCE2. No significant cross-reactivity or interference between human PCOLCE2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human PCOLCE2 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCOLCE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCOLCE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCOLCE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCOLCE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_037495.1
ncbi gb acc num :
NM_013363.3
ncbi mol weight :
45,717 Da
uniprot summary :
PCOLCE2: Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q21-q24. Molecular Function: heparin binding; collagen binding; protease activator activity
size4 :
10x96-Strip-Wells