catalog number :
MBS906981
products type :
ELISA Kit
products full name :
Mouse Mucin-19, MUC19 ELISA Kit
products short name :
mucin 19, oligomeric
products name syn :
Mouse Mucin-19 (MUC19) ELISA kit; FLJ35746; mucin 19; mucin 19; oligomeric
other names :
mucin-19; Mucin-19; mucin-19; sublingual apomucin; gel-forming secreted mucin-19; mucin 19; Gel-forming secreted mucin-19; Sublingual apomucin
products gene name :
MUC19
other gene names :
Muc19; Muc19; sld; MUC-19; MUC-19
uniprot entry name :
MUC19_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse MUC19. No significant cross-reactivity or interference between mouse MUC19 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of mouse mucin-19 (MUC19) concentrations in serum, plasma, tissue homogenates. Detection Range: 1.56 ng/ml-100 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of mouse MUC19 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC19 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_997126.2
ncbi gb acc num :
NM_207243.2
ncbi mol weight :
693,499 Da
ncbi pathways :
Metabolism Of Proteins Pathway 640416!!O-linked Glycosylation Of Mucins Pathway 640454!!Post-translational Protein Modification Pathway 640425!!Termination Of O-glycan Biosynthesis Pathway 640455
uniprot summary :
Function: May function in ocular mucus homeostasis