Human E3 ubiquitin-protein ligase MYLIP, MYLIP ELISA Kit | MyBioSource MBS906926 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human E3 ubiquitin-protein ligase MYLIP, MYLIP ELISA Kit

catalog :

MBS906926

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS906926

products type :

ELISA Kit

products full name :

Human E3 ubiquitin-protein ligase MYLIP, MYLIP ELISA Kit

products short name :

myosin regulatory light chain interacting protein

products name syn :

Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA kit; RP1-13D10.1; IDOL; MIR; band 4.1 superfamily member BZF1; cellular modulator of immune recognition (c-MIR) ; myosin regulatory light chain interacting protein

other names :

E3 ubiquitin-protein ligase MYLIP; E3 ubiquitin-protein ligase MYLIP; E3 ubiquitin-protein ligase MYLIP; band 4.1 superfamily member BZF1; inducible degrader of the LDL-receptor; cellular modulator of immune recognition (c-MIR); myosin regulatory light chain-interacting protein; E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor; myosin regulatory light chain interacting protein; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR

products gene name :

MYLIP

other gene names :

MYLIP; MYLIP; MIR; IDOL; BZF1; IDOL; BM-023; PP5242; Idol; MIR

uniprot entry name :

MYLIP_HUMAN

reactivity :

Human

sequence length :

445

specificity :

This assay has high sensitivity and excellent specificity for detection of Human MYLIP. No significant cross-reactivity or interference between Human MYLIP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Ubiquitin. Protein Biological Process 3: Ubl conjugation pathway

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38788243

ncbi acc num :

NP_037394.2

ncbi gb acc num :

NM_013262.3

uniprot acc num :

Q8WY64

ncbi mol weight :

49,910 Da

ncbi summary :

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

uniprot summary :

MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; Ubiquitin conjugating system; Motility/polarity/chemotaxis; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: cytoskeleton; extrinsic to membrane; cytoplasm; plasma membrane; intracellular. Molecular Function: protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage