ELISA/assay

Human Beta-galactoside alpha-2, 6-sialyltransferase 1, ST6GAL1 ELISA Kit

MBS906874

48-Strip-Wells

510 USD

ELISA Kit

Human Beta-galactoside alpha-2, 6-sialyltransferase 1, ST6GAL1 ELISA Kit

ST6 beta-galactosamide alpha-2, 6-sialyltranferase 1

Human Beta-galactoside alpha-2; 6-sialyltransferase 1 (ST6GAL1) ELISA kit; MGC48859; SIAT1; ST6GalI; ST6N; CMP-N-acetylneuraminate beta-galactosamide alpha-2; 6-sialyltransferase; OTTHUMP00000210002; ST6Gal I; alpha 2; 6-ST; sialyltransferase 1 (beta-galactoside alpha-2; 6-sialyl; ST6 beta-galactosamide alpha-2; 6-sialyltranferase 1

beta-galactoside alpha-2,6-sialyltransferase 1 isoform a; Beta-galactoside alpha-2,6-sialyltransferase 1; beta-galactoside alpha-2,6-sialyltransferase 1; ST6Gal I; alpha 2,6-ST 1; B-cell antigen CD75; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1

ST6GAL1

ST6GAL1; ST6GAL1; ST6N; SIAT1; ST6GalI; SIAT1; Alpha 2,6-ST 1; ST6GalI

SIAT1_HUMAN

Human

406

This assay has high sensitivity and excellent specificity for detection of human ST6GAL1. No significant cross-reactivity or interference between human ST6GAL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.9 ng/ml -250 ng/ml. Sensitivity: The minimum detectable dose of human ST6GAL1 is typically less than 0.97 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ST6GAL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ST6GAL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ST6GAL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ST6GAL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506949

NP_003023.1

NM_003032.2

P15907

46,605 Da

Asparagine N-linked Glycosylation Pathway (161013); Metabolism Of Proteins Pathway (106230); N-Glycan Antennae Elongation Pathway (187226); N-Glycan Biosynthesis Pathway (82975); N-Glycan Biosynthesis Pathway (345); N-glycan Antennae Elongation In The Medial/trans-Golgi Pathway (187223); N-glycan Biosynthesis, Complex Type Pathway (413356); N-glycan Biosynthesis, Complex Type Pathway (468278); O-linked Glycosylation Of Mucins Pathway (530747); Other Types Of O-glycan Biosynthesis Pathway (82978)

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]

SIAT1: a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. Is normally found in the trans cisternae of the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found. Protein type: Transferase; Membrane protein, integral; EC 2.4.99.1; Glycan Metabolism - N-glycan biosynthesis. Chromosomal Location of Human Ortholog: 3q27.3. Cellular Component: Golgi membrane; integral to Golgi membrane. Molecular Function: sialyltransferase activity; beta-galactoside alpha-2,6-sialyltransferase activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; humoral immune response; N-acetylneuraminate metabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800