catalog number :
MBS906717
products type :
ELISA Kit
products full name :
Mouse Syndecan-2, SDC2 ELISA Kit
products short name :
syndecan 2
products name syn :
Mouse Syndecan-2 (SDC2) ELISA kit; HSPG; HSPG1; SYND2; cell surface-associated heparan sulfate proteoglycan 1; fibroglycan; heparan sulfate proteoglycan 1; cell surface-associated; heparan sulfate proteoglycan core protein; syndecan prot; syndecan 2
other names :
syndecan-2; Syndecan-2; syndecan-2; HSPG; fibroglycan; heparan sulfate proteoglycan core protein; heparan sulfate proteoglycan 1, cell surface-associated (fibroglycan); syndecan 2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD_antigen: CD362
products gene name :
SDC2
other gene names :
Sdc2; Sdc2; Hspg1; Synd2; AA960457; syndecan-2; 4833414L08Rik; Hspg1; Synd2; SYND2; HSPG
uniprot entry name :
SDC2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SDC2. No significant cross-reactivity or interference between mouse SDC2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of mouse SDC2 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032330.1
ncbi gb acc num :
NM_008304.2
ncbi mol weight :
22,131 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Malaria Pathway (152666); Malaria Pathway (152657); TGF-beta Receptor Signaling Pathway (198330)
uniprot summary :
syndecan-2: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Preferentially expressed in cells of mesenchymal origin. Is tyrosine phosphorylated and forms a complex with EphB2 in mouse brain. Plays a role in mediating adhesion and proliferation of colon carcinoma cells. Protein type: Motility/polarity/chemotaxis; Cell surface; Cell adhesion; Membrane protein, integral. Cellular Component: cell soma; membrane; integral to membrane; synapse. Molecular Function: protein binding; PDZ domain binding. Biological Process: nervous system development; regulation of dendrite morphogenesis; cell differentiation
size4 :
10x96-Strip-Wells