ELISA/assay

Mouse Serine protease HTRA1, HTRA1 ELISA Kit

MBS906562

48-Strip-Wells

510 USD

ELISA Kit

Mouse Serine protease HTRA1, HTRA1 ELISA Kit

HtrA serine peptidase 1

Mouse Serine protease HTRA1 (HTRA1) ELISA kit; ARMD7; HtrA; L56; ORF480; PRSS11; IGFBP5-protease; protease; serine; 11 (IGF binding) ; HtrA serine peptidase 1

serine protease HTRA1; Serine protease HTRA1; serine protease HTRA1; serine protease 11; protease, serine, 11 (Igf binding); high-temperature requirement A serine peptidase 1; insulin-like growth factor binding protein 5 protease; HtrA serine peptidase 1; High-temperature requirement A serine peptidase 1; Serine protease 11

HTRA1

Htra1; Htra1; L56; HTRA; Prss11; RSPP11; AI429470; Htra; Prss11

HTRA1_MOUSE

Mouse

480

This assay has high sensitivity and excellent specificity for detection of mouse HTRA1. No significant cross-reactivity or interference between mouse HTRA1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTRA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTRA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTRA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTRA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

229093139

NP_062510.2

NM_019564.3

Q9R118

51,214 Da

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family. Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted. Cellular Component: extracellular matrix; cytoplasm; extracellular region. Molecular Function: peptidase activity; insulin-like growth factor binding; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; growth factor binding; catalytic activity. Biological Process: regulation of cell growth; negative regulation of defense response to virus; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800