Human eosinophil granule major basic protein, EMBP/MBP ELISA Kit | MyBioSource MBS906454 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human eosinophil granule major basic protein, EMBP/MBP ELISA Kit

catalog :

MBS906454

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS906454

products type :

ELISA Kit

products full name :

Human eosinophil granule major basic protein, EMBP/MBP ELISA Kit

products short name :

proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)

products name syn :

Human eosinophil granule major basic protein (EMBP/MBP) ELISA Kit; BMPG; MBP; MBP1; MGC14537; bone-marrow proteoglycan; eosinophil granule major basic protein; eosinophil major basic protein; natural killer cell activator; proteoglycan 2; proteoglycan 2 preproprotein; proteoglycan 2; bone marrow (natural killer cell activator; eosinophil granule major basic protein)

other names :

bone marrow proteoglycan isoform 2 preproprotein; Bone marrow proteoglycan; bone marrow proteoglycan; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein; proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein); Proteoglycan 2Cleaved into the following chain:Eosinophil granule major basic protein; EMBP; MBPAlternative name(s):Pregnancy-associated major basic protein

products gene name :

PRG2

other gene names :

PRG2; PRG2; MBP; BMPG; MBP1; MBP; BMPG; EMBP; MBP

uniprot entry name :

PRG2_HUMAN

reactivity :

Human

sequence length :

211

specificity :

This assay has high sensitivity and excellent specificity for detection of human EMBP. No significant cross-reactivity or interference between human EMBP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 1.17 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EMBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EMBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EMBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

342837699

ncbi acc num :

NP_001230174.1

ncbi gb acc num :

NM_001243245.1

uniprot acc num :

P13727

ncbi mol weight :

25,206 Da

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. Protein type: Secreted, signal peptide; Secreted; Vesicle. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: transport vesicle; extracellular region. Molecular Function: heparin binding; carbohydrate binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage