catalog number :
MBS906206
products type :
ELISA Kit
products full name :
Pig Pancreatic triacylglycerol lipase, PNLIP ELISA Kit
products short name :
pancreatic lipase
products name syn :
Pig Pancreatic triacylglycerol lipase (PNLIP) ELISA kit; PL; pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase; pancreatic lipase
other names :
Pancreatic triacylglycerol lipase; Pancreatic triacylglycerol lipase
products gene name :
PNLIP
other gene names :
PNLIP; PL; PTL; Pancreatic lipase
uniprot entry name :
LIPP_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig PNLIP. No significant cross-reactivity or interference between pig PNLIP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.625 mU/ml-40 mU/ml. Sensitivity: The minimum detectable dose of pig PNLIP is typically less than 0.156 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PNLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PNLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNLIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
50,084 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells