catalog number :
MBS906130
products type :
ELISA Kit
products full name :
Mouse Follistatin-related protein 1, FSTL1 ELISA Kit
products short name :
follistatin-like 1
products name syn :
Mouse Follistatin-related protein 1 (FSTL1) ELISA kit; FLJ50214; FLJ52277; FRP; FSL1; follistatin-related protein; follistatin-like 1
other names :
follistatin-related protein 1; Follistatin-related protein 1; follistatin-related protein 1; follistatin-like protein 1; TGF-beta-inducible protein TSC-36; follistatin-like 1; Follistatin-like protein 1; TGF-beta-inducible protein TSC-36
products gene name :
FSTL1
other gene names :
Fstl1; Fstl1; Fstl; TSC-36; AI316791; AW107808; Frp; Fstl; Tsc36
uniprot entry name :
FSTL1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse FSTL1. No significant cross-reactivity or interference between mouse FSTL1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.18 ng/ml -12 ng/ml. Sensitivity: The minimum detectable dose of mouse FSTL1 is typically less than 0.04 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSTL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032073.2
ncbi gb acc num :
NM_008047.5
ncbi mol weight :
34,554 Da
ncbi pathways :
Signal Transduction Pathway (575318); Signaling By BMP Pathway (575413)
uniprot summary :
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; calcium ion binding. Biological Process: response to starvation
size4 :
10x96-Strip-Wells