Human Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit | MyBioSource MBS906051 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit

catalog :

MBS906051

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS906051

products type :

ELISA Kit

products full name :

Human Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit

products short name :

[cholinergic receptor, muscarinic 3]

products name syn :

[Human Muscarinic acetylcholine receptor M3 (CHRM3) ELISA kit; HM3; m3 muscarinic receptor; muscarinic acetylcholine receptor M3; cholinergic receptor; muscarinic 3]

other names :

[muscarinic acetylcholine receptor M3; Muscarinic acetylcholine receptor M3; muscarinic acetylcholine receptor M3; m3 muscarinic receptor; acetylcholine receptor, muscarinic 3; cholinergic receptor, muscarinic 3]

products gene name :

[CHRM3]

other gene names :

[CHRM3; CHRM3; HM3; EGBRS]

uniprot entry name :

ACM3_HUMAN

reactivity :

Human

sequence length :

590

specificity :

This assay has high sensitivity and excellent specificity for detection of human CHRM3. No significant cross-reactivity or interference between human CHRM3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 5.86 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502819

ncbi acc num :

NP_000731.1

ncbi gb acc num :

NM_000740.2

uniprot acc num :

P20309

ncbi mol weight :

66,128 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cholinergic Synapse Pathway (217716); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

ncbi summary :

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

uniprot summary :

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction. Molecular Function: protein binding; drug binding; receptor activity; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity. Biological Process: nervous system development; G-protein coupled receptor protein signaling pathway; cell proliferation; acetylcholine receptor signaling, muscarinic pathway; smooth muscle contraction; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion. Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage