Mouse N-glycosylase/DNA lyase, OGG1 ELISA Kit | MyBioSource MBS906002 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse N-glycosylase/DNA lyase, OGG1 ELISA Kit

catalog :

MBS906002

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS906002

products type :

ELISA Kit

products full name :

Mouse N-glycosylase/DNA lyase, OGG1 ELISA Kit

products short name :

N-glycosylase/DNA lyase (OGG1)

products name syn :

Mouse N-glycosylase/DNA lyase (OGG1) ELISA kit; HMMH; HOGG1; MUTM; OGH1; 8-hydroxyguanine DNA glycosylase; 8-oxoguanine DNA-glycosylase 1; AP lyase; DNA-apurinic or apyrimidinic site lyase; N-glycosylase/DNA lyase (OGG1)

other names :

N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; 8-oxoguanine DNA-glycosylase 1

products gene name :

OGG1

other gene names :

Ogg1; Ogg1; Mmh; AP lyase

uniprot entry name :

OGG1_MOUSE

reactivity :

Mouse

sequence length :

345

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse OGG1. No significant cross-reactivity or interference between mouse OGG1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 43.75 pg/ml -2800 pg/ml. Sensitivity: 10.93 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OGG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OGG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OGG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OGG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

187960092

ncbi acc num :

NP_035087.3

ncbi gb acc num :

NM_010957.4

uniprot acc num :

O08760

ncbi mol weight :

38,883 Da

ncbi pathways :

Base Excision Repair Pathway (574959); Base Excision Repair Pathway (83240); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (574960); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (574969); Cleavage Of The Damaged Purine Pathway (574963); DNA Repair Pathway (574958); Depurination Pathway (574961); Displacement Of DNA Glycosylase By APE1 Pathway (574970); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (574962)

uniprot summary :

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3 to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: DNA repair, damage; Deoxyribonuclease; Lyase; EC 4.2.99.18. Cellular Component: nucleoplasm; protein complex; nuclear matrix; mitochondrion; nuclear speck; nucleus. Molecular Function: single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; hydrolase activity; microtubule binding; DNA N-glycosylase activity; hydrolase activity, acting on glycosyl bonds; lyase activity; purine-specific mismatch base pair DNA N-glycosylase activity; DNA-3-methyladenine glycosylase III activity; DNA binding; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; oxidized base lesion DNA N-glycosylase activity; catalytic activity; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity. Biological Process: metabolic process; nucleotide-excision repair, DNA incision; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; regulation of transcription, DNA-dependent; nucleotide-excision repair; base-excision repair; regulation of protein import into nucleus, translocation; response to oxidative stress; response to DNA damage stimulus; aging; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage