Mouse Lymphoid enhancer-binding factor 1, LEF1 ELISA Kit | MyBioSource MBS905884 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Lymphoid enhancer-binding factor 1, LEF1 ELISA Kit

catalog :

MBS905884

quantity :

48-Strip-Wells

price :

510 USD

product information

catalog number :

MBS905884

products type :

ELISA Kit

products full name :

Mouse Lymphoid enhancer-binding factor 1, LEF1 ELISA Kit

products short name :

lymphoid enhancer-binding factor 1

products name syn :

Mouse Lymphoid enhancer-binding factor 1 (LEF1) ELISA kit; DKFZp586H0919; FLJ46390; TCF1ALPHA; T cell-specific transcription factor 1-alpha; TCF1-alpha; lymphoid enhancer-binding factor 1

other names :

lymphoid enhancer-binding factor 1 isoform a; Lymphoid enhancer-binding factor 1; lymphoid enhancer-binding factor 1; lymphoid enhancer binding factor 1

products gene name :

LEF1

other gene names :

Lef1; Lef1; Lef-1; AI451430; 3000002B05; Lef-1; LEF-1

uniprot entry name :

LEF1_MOUSE

reactivity :

Mouse

sequence length :

397

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse LEF1. No significant cross-reactivity or interference between mouse LEF1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: The minimum detectable dose of mouse LEF1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LEF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27735019

ncbi acc num :

NP_034833.2

ncbi gb acc num :

NM_010703.4

uniprot acc num :

P27782

ncbi mol weight :

44,059 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83310); Acute Myeloid Leukemia Pathway (529); Adherens Junction Pathway (83267); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117303); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Colorectal Cancer Pathway (83299); Colorectal Cancer Pathway (518)

uniprot summary :

LEF-1: a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Protein type: Transcription factor; DNA-binding. Cellular Component: transcription factor complex; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; estrogen receptor activity; histone binding; caspase inhibitor activity; beta-catenin binding; transcription factor binding; protein binding; DNA binding; sequence-specific DNA binding; gamma-catenin binding; estrogen receptor binding; chromatin binding; DNA bending activity; transcription factor activity. Biological Process: radial glial cell differentiation in the forebrain; hypothalamus development; positive regulation of transcription, DNA-dependent; T-helper 1 cell differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain neuroblast division; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; neural crest cell migration; embryonic limb morphogenesis; somitogenesis; dentate gyrus development; transcription, DNA-dependent; sensory perception of taste; negative regulation of striated muscle development; positive regulation of cell growth; patterning of blood vessels; regulation of transcription from RNA polymerase II promoter; negative regulation of interleukin-5 production; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; B cell proliferation; transcription from RNA polymerase II promoter; tongue development; negative regulation of interleukin-4 production; paraxial mesoderm formation; positive regulation of granulocyte differentiation; alpha-beta T cell differentiation; negative regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of caspase activity; anatomical structure regression; regulation of cell-cell adhesion; regulation of transcription, DNA-dependent; vasculature development; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; Wnt receptor signaling pathway; neutrophil differentiation; hippocampus development; regulation of Wnt receptor signaling pathway; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; osteoblast differentiation; formation of radial glial scaffolds; T cell receptor V(D)J recombination; epithelial to mesenchymal transition; negative regulation of cell-cell adhesion; sprouting angiogenesis; cell development; eye pigmentation; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800