ELISA/assay

Rat choline acetyltransterase, ChAT ELISA Kit

MBS905873

48-Strip-Wells

565 USD

ELISA Kit

Rat choline acetyltransterase, ChAT ELISA Kit

choline acetyltransterase (ChAT)

Rat choline acetyltransterase (ChAT) ELISA Kit; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase; choline acetyltransterase (ChAT)

Choline O-acetyltransferase; Choline O-acetyltransferase; choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; choline O-acetyltransferase

CHAT

Chat; Chat; CHOACTase; ChAT; Choline acetylase

CLAT_RAT

Rat

640

This assay has high sensitivity and excellent specificity for detection of Rat CHAT. No significant cross-reactivity or interference between Rat CHAT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Neurotransmitter biosynthesis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ChAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ChAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ChAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ChAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

416805

P32738.2

P32738

71,864 Da

Acetylcholine Neurotransmitter Release Cycle Pathway (574443); Biogenic Amine Synthesis Pathway (198489); Cholinergic Synapse Pathway (217713); Glycerophospholipid Metabolism Pathway (83381); Glycerophospholipid Metabolism Pathway (364); Neuronal System Pathway (574433); Neurotransmitter Release Cycle Pathway (574438); Transmission Across Chemical Synapses Pathway (574436)

CHAT: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Protein type: EC 2.3.1.6; Acetyltransferase; Lipid Metabolism - glycerophospholipid. Cellular Component: neuron projection; mitochondrion; cell soma; axon; cytoplasm. Molecular Function: choline binding; choline O-acetyltransferase activity. Biological Process: response to drug; muscle development; rhythmic excitation; metabolic process; antral ovarian follicle growth; adult walking behavior; memory; neuron differentiation; synaptic transmission; rhythmic behavior; response to ethanol; acetylcholine biosynthetic process; response to hypoxia; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; response to nutrient; neurotransmitter biosynthetic process

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150