Human Soluble CD276, sCD276/sB7-H3 ELISA Kit | MyBioSource MBS905704 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Soluble CD276, sCD276/sB7-H3 ELISA Kit

catalog :

MBS905704

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS905704

products type :

ELISA Kit

products full name :

Human Soluble CD276, sCD276/sB7-H3 ELISA Kit

products short name :

CD276 molecule

products name syn :

Human Soluble CD276; sCD276/sB7-H3 ELISA Kit; B7-H3; B7H3; B7 homolog 3; CD276 antigen; CD276 molecule

other names :

CD276 antigen isoform a; CD276 antigen; CD276 antigen; B7 homolog 3; costimulatory molecule; CD276 molecule; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD_antigen: CD276

products gene name :

CD276

other gene names :

CD276; CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; B7H3; PSEC0249; UNQ309/PRO352; B7-H3

uniprot entry name :

CD276_HUMAN

reactivity :

Human

sequence length :

534

specificity :

This assay has high sensitivity and excellent specificity for detection of human sCD276/sB7-H3. No significant cross-reactivity or interference between human sCD276/sB7-H3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human sCD276/sB7-H3 is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD276/sB7-H3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD276/sB7-H3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD276/sB7-H3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD276/sB7-H3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

67188443

ncbi acc num :

NP_001019907.1

ncbi gb acc num :

NM_001024736.1

uniprot acc num :

Q5ZPR3

ncbi mol weight :

57,235 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 15q23-q24. Cellular Component: integral to membrane; external side of plasma membrane. Molecular Function: protein binding; receptor binding. Biological Process: positive regulation of osteoblast differentiation; cell proliferation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; immune response; positive regulation of T cell proliferation; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma biosynthetic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage