catalog number :
MBS905558
products type :
ELISA Kit
products full name :
Human Alpha-defensin 7, DEFA7 ELISA Kit
products short name :
defensin, alpha 7
products name syn :
Human Alpha-defensin 7 (DEFA7) ELISA kit; defensin; alpha 7
other names :
Alpha-defensin 7; Alpha-defensin 7; Defensin-related cryptdin-7
products gene name :
DEFA7
other gene names :
Defa7; Defcr7
uniprot entry name :
DEFA7_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of human DEFA7. No significant cross-reactivity or interference between human DEFA7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human DEFA7 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
10,496 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
size4 :
10x96-Strip-Wells