Mouse Translationally-controlled tumor protein, TPT1 ELISA Kit | MyBioSource MBS905544 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Translationally-controlled tumor protein, TPT1 ELISA Kit

catalog :

MBS905544

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS905544

products type :

ELISA Kit

products full name :

Mouse Translationally-controlled tumor protein, TPT1 ELISA Kit

products short name :

tumor protein, translationally-controlled 1

products name syn :

Mouse Translationally-controlled tumor protein (TPT1) ELISA kit; FLJ27337; HRF; TCTP; p02; fortilin; histamine-releasing factor; tumor protein; translationally-controlled 1

other names :

translationally-controlled tumor protein; Translationally-controlled tumor protein; translationally-controlled tumor protein; fortilin; translationally regulated transcript (21 kDa); 21 kDa polypeptide under translational control; tumor protein, translationally-controlled 1; 21 kDa polypeptide; p21; p23

products gene name :

TPT1

other gene names :

Tpt1; Tpt1; Trt; p21; p23; TCTP; Trt; TCTP

uniprot entry name :

TCTP_MOUSE

reactivity :

Mouse

sequence length :

172

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse TPT1. No significant cross-reactivity or interference between mouse TPT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 9.37 pg/ml -600 pg/ml. Sensitivity: The minimum detectable dose of mouse TPT1 is typically less than 2.34 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6678437

ncbi acc num :

NP_033455.1

ncbi gb acc num :

NM_009429.3

uniprot acc num :

P63028

ncbi mol weight :

19,462 Da

uniprot summary :

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation. Protein type: Microtubule-binding; Calcium-binding. Cellular Component: nucleoplasm; multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus. Molecular Function: calcium ion binding; transcription factor binding. Biological Process: stem cell maintenance

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage