Rat Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit | MyBioSource MBS905355 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit

catalog :

MBS905355

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS905355

products type :

ELISA Kit

products full name :

Rat Muscarinic acetylcholine receptor M3, CHRM3 ELISA Kit

products short name :

cholinergic receptor, muscarinic 3

products name syn :

Rat Muscarinic acetylcholine receptor M3 (CHRM3) ELISA kit; HM3; m3 muscarinic receptor; muscarinic acetylcholine receptor M3; cholinergic receptor; muscarinic 3

other names :

muscarinic acetylcholine receptor M3; Muscarinic acetylcholine receptor M3; muscarinic acetylcholine receptor M3; cholinergic receptor, muscarinic 3, cardiac; cholinergic receptor, muscarinic 3

products gene name :

CHRM3

other gene names :

Chrm3; Chrm3; Chrm-3

uniprot entry name :

ACM3_RAT

reactivity :

Rat

sequence length :

589

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat CHRM3. No significant cross-reactivity or interference between Rat CHRM3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

451172073

ncbi acc num :

NP_036659.2

ncbi gb acc num :

NM_012527.2

uniprot acc num :

P08483

ncbi mol weight :

66,066 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (574231); Calcium Regulation In The Cardiac Cell Pathway (198495); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Cholinergic Synapse Pathway (217713); Class A/1 (Rhodopsin-like Receptors) Pathway (574224); G Alpha (q) Signalling Events Pathway (574254); GPCR Downstream Signaling Pathway (574250); GPCR Ligand Binding Pathway (574223); GPCRs, Class A Rhodopsin-like Pathway (198500)

ncbi summary :

G-protein coupled receptor, muscarinic receeptor subtype that mediates cholinergic-induced contraction in most smooth muscle [RGD, Feb 2006]

uniprot summary :

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR. Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction. Molecular Function: protein binding; drug binding; acetylcholine binding; G-protein coupled acetylcholine receptor activity. Biological Process: acetylcholine receptor signaling, muscarinic pathway; smooth muscle contraction; regulation of vasoconstriction; regulation of vascular smooth muscle contraction; digestion; positive regulation of smooth muscle contraction; saliva secretion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage