ELISA/assay

Human Protein FAM3C, FAM3C ELISA Kit

MBS905346

48-Strip-Wells

510 USD

ELISA Kit

Human Protein FAM3C, FAM3C ELISA Kit

family with sequence similarity 3, member C

Human Protein FAM3C (FAM3C) ELISA kit; GS3786; ILEI; interleukin-like EMT inducer; predicted osteoblast protein; family with sequence similarity 3; member C

protein FAM3C; Protein FAM3C; protein FAM3C; interleukin-like EMT inducer; predicted osteoblast protein; interleukin-like epithelial-mesenchymal transition inducer; family with sequence similarity 3, member C; Interleukin-like EMT inducer

FAM3C

FAM3C; FAM3C; ILEI; GS3786; ILEI; GS3786

FAM3C_HUMAN

Human

227

This assay has high sensitivity and excellent specificity for detection of human FAM3C. No significant cross-reactivity or interference between human FAM3C and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human FAM3C is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM3C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM3C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM3C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3C bound in the initial step. The color development is stopped and the intensity of the color is measured.

91807125

NP_001035109.1

NM_001040020.1

Q92520

24,680 Da

This gene is a member of the family with sequence similarity 3 (FAM3) family and encodes a secreted protein with a GG domain. A change in expression of this protein has been noted in pancreatic cancer-derived cells. [provided by RefSeq, Mar 2010]

FAM3C: May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. Belongs to the FAM3 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q31. Cellular Component: Golgi apparatus; cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: cytokine activity. Biological Process: multicellular organismal development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800