ELISA/assay

Human Angiopoietin-1, ANGPT1 ELISA Kit

MBS905339

96-Strip-Wells

680 USD

ELISA Kit

Human Angiopoietin-1, ANGPT1 ELISA Kit

angiopoietin 1

Human Angiopoietin-1 (ANGPT1) ELISA kit; AGP1; AGPT; ANG1; ; Human Angiopoietin-1 (ANGPT1)

angiopoietin-1 isoform 1; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1

ANGPT1

ANGPT1; ANGPT1; AGP1; AGPT; ANG1; KIAA0003; ANG-1

ANGP1_HUMAN

Human

498

This assay has high sensitivity and excellent specificity for detection of Human ANGPT1. No significant cross-reactivity or interference between Human ANGPT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.31 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

20532340

NP_001137.2

NM_001146.3

Q15389

57,513 Da

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); Tie2 Signaling Pathway (106063)

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; microvillus; extracellular region; plasma membrane; lipid raft. Molecular Function: receptor tyrosine kinase binding. Biological Process: positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; positive chemotaxis; negative regulation of protein amino acid phosphorylation; hemopoiesis; negative regulation of neuron apoptosis; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; regulation of endothelial cell proliferation; Tie receptor signaling pathway; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; blood coagulation; leukocyte migration; negative regulation of apoptosis

96-Strip-Wells

680 USD

5x96-Strip-Wells

2140

10x96-Strip-Wells

3990