catalog number :
MBS905143
products type :
ELISA Kit
products full name :
Human Mucin-3B, MUC3B ELISA Kit
products short name :
mucin 3B, cell surface associated
products name syn :
Human Mucin-3B (MUC3B) ELISA kit; MUC3; MUC3A; intestinal mucin MUC3B; mucin 3B; mucin 3B; cell surface associated
other names :
Mucin-3B; Mucin-3B; mucin-3B; MUC-3B; intestinal mucin-3B; intestinal mucin MUC3B; mucin 3B, cell surface associated; Intestinal mucin-3B
products gene name :
MUC3B
other gene names :
MUC3B; MUC3B; MUC3; MUC3A; MUC-3B
uniprot entry name :
MUC3B_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MUC3B. No significant cross-reactivity or interference between human MUC3B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human MUC3B is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
131,402 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)
uniprot summary :
MUC3B: Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: Golgi lumen; integral to membrane. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification
size4 :
10x96-Strip-Wells