ELISA/assay

Human DNA dC->dU-editing enzyme APOBEC-3G, APOBEC3G ELISA Kit

MBS905122

48-Strip-Wells

510 USD

ELISA Kit

Human DNA dC->dU-editing enzyme APOBEC-3G, APOBEC3G ELISA Kit

apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G

Human DNA dC->dU-editing enzyme APOBEC-3G (APOBEC3G) ELISA kit; ARP9; CEM15; FLJ12740; MDS019; bK150C2.7; dJ494G10.1; DNA dC->dU editing enzyme; phorbolin-like protein MDS019; apolipoprotein B mRNA editing enzyme; catalytic polypeptide-like 3G

DNA dC- dU-editing enzyme APOBEC-3G; DNA dC->dU-editing enzyme APOBEC-3G; DNA dC- dU-editing enzyme APOBEC-3G; deoxycytidine deaminase; APOBEC-related protein 9; DNA dC- dU editing enzyme; phorbolin-like protein MDS019; APOBEC-related cytidine deaminase; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3G; apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G

APOBEC3G

APOBEC3G; APOBEC3G; A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1; MDS019; APOBEC-related protein; ARCD; ARP-9; CEM15; A3G

ABC3G_HUMAN

Human

384

This assay has high sensitivity and excellent specificity for detection of human APOBEC3G. No significant cross-reactivity or interference between human APOBEC3G and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 6.25 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOBEC3G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOBEC3G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOBEC3G is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOBEC3G bound in the initial step. The color development is stopped and the intensity of the color is measured.

13399304

NP_068594.1

NM_021822.3

Q9HC16

46,408 Da

APOBEC3G Mediated Resistance To HIV-1 Infection Pathway (106016); Disease Pathway (530764); HIV Infection Pathway (105983); Host Interactions Of HIV Factors Pathway (106010); Vif-mediated Degradation Of APOBEC3G Pathway (106017)

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Jul 2008]

APOBEC3G: DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. May also prevent the transposition of a subset of retroelements. Binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; RNA-binding; EC 3.5.4.-; RNA processing. Chromosomal Location of Human Ortholog: 22q13.1-q13.2. Cellular Component: cytoplasm; apolipoprotein B mRNA editing enzyme complex; cytosol; ribonucleoprotein complex. Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; deoxycytidine deaminase activity; RNA binding; cytidine deaminase activity. Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; viral reproduction; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800