catalog number :
MBS905104
products type :
ELISA Kit
products full name :
Rat Alpha-crystallin A chain, CRYAA ELISA Kit
products short name :
crystallin, alpha A
products name syn :
Rat Alpha-crystallin A chain (CRYAA) ELISA kit; CRYA1; HSPB4; crystallin; alpha-1; human alphaA-crystallin (CRYA1) ; crystallin; alpha A
other names :
alpha-crystallin A chain; Alpha-crystallin A chain; alpha-crystallin A chain; alpha-A-crystallin
products gene name :
CRYAA
other gene names :
CRYAA; CRYAA
uniprot entry name :
CRYAA_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CRYAA. No significant cross-reactivity or interference between rat CRYAA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 39 pg/ml-2500 pg/ml. Sensitivity: The minimum detectable dose of rat CRYAA is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRYAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRYAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRYAA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRYAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075875.2
ncbi gb acc num :
NM_001082406.2
ncbi mol weight :
19,837 Da
uniprot summary :
CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens. Protein type: Heat shock protein; Chaperone
size4 :
10x96-Strip-Wells