catalog number :
MBS905093
products type :
ELISA Kit
products full name :
Rat Beta-hexosaminidase subunit alpha, HEXA ELISA Kit
products short name :
hexosaminidase A (alpha polypeptide)
products name syn :
Rat Beta-hexosaminidase subunit alpha (HEXA) ELISA kit; MGC99608; TSD; N-acetyl-beta-glucosaminidase; beta-N-acetylhexosaminidase; hexosaminidase A; hexosaminidase A (alpha polypeptide)
other names :
beta-hexosaminidase subunit alpha; Beta-hexosaminidase subunit alpha; beta-hexosaminidase subunit alpha; hexosaminidase subunit A; Hexose aminidase A (alpha polypeptide); beta-N-acetylhexosaminidase subunit alpha; N-acetyl-beta-glucosaminidase subunit alpha; hexosaminidase A; Beta-N-acetylhexosaminidase subunit alpha; Hexosaminidase subunit A; N-acetyl-beta-glucosaminidase subunit alpha
products gene name :
HEXA
other gene names :
Hexa; Hexa; Hexosaminidase subunit A
uniprot entry name :
HEXA_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat HEXA. No significant cross-reactivity or interference between rat HEXA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 1.56 mU/ml -100 mU/ml. Sensitivity: 0.39 mU/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HEXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEXA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEXA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001004443.1
ncbi gb acc num :
NM_001004443.1
ncbi mol weight :
60,538 Da
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway (83371); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Glycosaminoglycan Degradation Pathway (83373); Glycosaminoglycan Degradation Pathway (355); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (83389); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (372); Glycosphingolipid Biosynthesis - Globo Series Pathway (83388); Glycosphingolipid Biosynthesis - Globo Series Pathway (371); Keratan Sulfate Degradation Pathway (434694); Keratan Sulfate Degradation Pathway (468269)
ncbi summary :
mouse homolog is the alpha subunit of lysosomal beta-hexosaminidase A [RGD, Feb 2006]
uniprot summary :
Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues . Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
size4 :
10x96-Strip-Wells