catalog number :
MBS905088
products type :
ELISA Kit
products full name :
Mouse Epidermal growth factor-like protein 6, EGFL6 ELISA Kit
products short name :
EGF-like-domain, multiple 6
products name syn :
Mouse Epidermal growth factor-like protein 6 (EGFL6) ELISA kit; DKFZp564P2063; MAEG; W80; EGF repeat-containing protein 6; MAM and EGF domain containing; epidermal growth factor-like protein 6; EGF-like-domain; multiple 6
other names :
epidermal growth factor-like protein 6; Epidermal growth factor-like protein 6; epidermal growth factor-like protein 6; EGF-like protein 6; MAM and EGF domains-containing gene protein; EGF-like-domain, multiple 6; MAM and EGF domains-containing gene protein; Protein W80
products gene name :
EGFL6
other gene names :
Egfl6; Egfl6; Maeg; Maeg; EGF-like protein 6
uniprot entry name :
EGFL6_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse EGFL6. No significant cross-reactivity or interference between Mouse EGFL6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 93.75 pg/ml-6000 pg/ml. Sensitivity: 23.43 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062270.1
ncbi gb acc num :
NM_019397.3
ncbi mol weight :
61,520 Da
uniprot summary :
EGFL6: May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: proteinaceous extracellular matrix; membrane; extracellular region; basement membrane. Molecular Function: calcium ion binding. Biological Process: extracellular matrix organization and biogenesis; multicellular organismal development; cell adhesion; cell differentiation
size4 :
10x96-Strip-Wells