catalog number :
MBS905052
products type :
ELISA Kit
products full name :
Horse Cysteine-rich secretory protein 3, CRISP3 ELISA Kit
products short name :
cysteine-rich secretory protein 3
products name syn :
Horse Cysteine-rich secretory protein 3 (CRISP3) ELISA kit; Aeg2; CRISP-3; CRS3; MGC126588; SGP28; dJ442L6.3; OTTHUMP00000016590; cysteine-rich secretory protein-3; specific granule protein (28 kDa) ; cysteine-rich secretory protein 3
other names :
cysteine-rich secretory protein 3; Cysteine-rich secretory protein 3; cysteine-rich secretory protein 3; CRISP-3; horse seminal plasma protein 3; acidic epididymal glycoprotein 2; Acidic epididymal glycoprotein 2; AEG2
products gene name :
CRISP3
other gene names :
CRISP3; CRISP3; AEG2; HSP-3; CRISP-3; AEG2
uniprot entry name :
CRIS3_HORSE
specificity :
This assay has high sensitivity and excellent specificity for detection of horse CRISP3. No significant cross-reactivity or interference between horse CRISP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.156 ng/ml - 10 ng /ml. Sensitivity: The minimum detectable dose of horse CRISP3 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CRISP3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for CRISP3. The competitive inhibition reaction is launched between with pre-coated CRISP3 and CRISP3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of CRISP3 in the samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075343.1
ncbi gb acc num :
NM_001081874.1
ncbi mol weight :
27,308 Da
size4 :
10x96-Strip-Wells