catalog number :
MBS904975
products type :
ELISA Kit
products full name :
Chicken Lipopolysaccharide-induced tumor necrosis factor-alpha factor, LITAF ELISA Kit
products short name :
lipopolysaccharide-induced TNF factor
products name syn :
Chicken Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) ELISA kit; FLJ38636; MGC116698; MGC116700; MGC116701; MGC125274; MGC125275; MGC125276; PIG7; SIMPLE; TP53I7; LPS-induced TNF-alpha factor; lipopolysaccharide-induced TNF-alpha factor; lipopolysaccharide-induced; lipopolysaccharide-induced TNF factor
other names :
lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; TNF-alpha factor; tissue necrosis factor-alpha; lipid-induced TNF-alpha factor; LPS-induced TNF-alpha factor homolog; small integral membrane protein of lysosome/late endosome; lipopolysaccharide-induced TNF factor; Small integral membrane protein of lysosome/late endosome
products gene name :
LITAF
other gene names :
LITAF; LITAF; TNF-alpha; SIMPLE; LPS-induced TNF-alpha factor homolog
uniprot entry name :
LITAF_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Chicken LITAF. No significant cross-reactivity or interference between Chicken LITAF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LITAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LITAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LITAF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LITAF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_989598.1
ncbi gb acc num :
NM_204267.1
ncbi mol weight :
16,013 Da
uniprot summary :
Function: Probable role in regulating transcription of specific genes
size4 :
10x96-Strip-Wells