Human Reelin, RELN ELISA Kit | MyBioSource MBS904954 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Reelin, RELN ELISA Kit

catalog :

MBS904954

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS904954

products type :

ELISA Kit

products full name :

Human Reelin, RELN ELISA Kit

products short name :

[reelin]

products name syn :

[Human Reelin (RELN) ELISA kit; PRO1598; RL; OTTHUMP00000211830; reelin]

other names :

[reelin isoform a; Reelin; reelin; reelin]

products gene name :

[RELN]

other gene names :

[RELN; RELN; RL; LIS2; PRO1598]

uniprot entry name :

RELN_HUMAN

reactivity :

Human

sequence length :

3460

specificity :

This assay has high sensitivity and excellent specificity for detection of human RELN. No significant cross-reactivity or interference between human RELN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: Typically less than 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27436938

ncbi acc num :

NP_005036.2

ncbi gb acc num :

NM_005045.3

uniprot acc num :

P78509

ncbi mol weight :

388,388 Da

ncbi pathways :

ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway (137984); Reelin Signaling Pathway (137980)

ncbi summary :

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Cell adhesion; Protease; EC 3.4.21.-; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: proteinaceous extracellular matrix; extracellular space; dendrite; cytoplasm. Molecular Function: serine-type peptidase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity. Biological Process: axon guidance; cellular morphogenesis during differentiation; peptidyl-tyrosine phosphorylation; central nervous system development; spinal cord patterning; neuron migration; response to pain; proteolysis; positive regulation of synaptic transmission, glutamatergic; glial cell differentiation; dendrite development; cell adhesion; positive regulation of TOR signaling pathway; associative learning; layer formation in the cerebral cortex; regulation of behavior; regulation of synaptic transmission; cerebral cortex tangential migration; hippocampus development; activation of CREB transcription factor; ventral spinal cord development; positive regulation of small GTPase mediated signal transduction; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; long-term memory; positive regulation of protein kinase activity; brain development. Disease: Epilepsy, Familial Temporal Lobe, 7; Lissencephaly 2; Epilepsy, Familial Temporal Lobe, 1

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145