catalog number :
MBS904839
products type :
ELISA Kit
products full name :
Rat Uromodulin, UMOD ELISA Kit
products short name :
uromodulin
products name syn :
Rat Uromodulin (UMOD) ELISA kit; ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP; Tamm-Horsfall glycoprotein; uromodulin (uromucoid; Tamm-Horsfall glycoprotein) ; uromucoid; uromodulin
other names :
uromodulin; Uromodulin; uromodulin; Urmodulin (Tamm-Horsfall protein); tamm-Horsfall urinary glycoprotein; uromodulin; Tamm-Horsfall urinary glycoprotein; THP
products gene name :
UMOD
other gene names :
Umod; Umod; THP; THP
uniprot entry name :
UROM_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat UMOD. No significant cross-reactivity or interference between Rat UMOD and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UMOD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UMOD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UMOD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UMOD bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_058778.1
ncbi gb acc num :
NM_017082.1
ncbi mol weight :
71,062 Da
ncbi summary :
mutation of the human homolog is associated with medullary cystic kidney disease 2 and familial juvenile hyperuricaemic nephropathy [RGD, Feb 2006]
uniprot summary :
Function: Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial
size4 :
10x96-Strip-Wells