Human Lysyl oxidase homolog 2, LOXL2 ELISA Kit | MyBioSource MBS904757 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lysyl oxidase homolog 2, LOXL2 ELISA Kit

catalog :

MBS904757

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904757

products type :

ELISA Kit

products full name :

Human Lysyl oxidase homolog 2, LOXL2 ELISA Kit

products short name :

lysyl oxidase-like 2

products name syn :

Human Lysyl oxidase homolog 2 (LOXL2) ELISA kit; LOR2; WS9-14; lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2

other names :

lysyl oxidase homolog 2; Lysyl oxidase homolog 2; lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2 protein; lysyl oxidase-like protein 2; lysyl oxidase-like 2 delta e13; lysyl oxidase-related protein 2; lysyl oxidase-related protein WS9-14; lysyl oxidase-like 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14

products gene name :

LOXL2

other gene names :

LOXL2; LOXL2; LOR2; WS9-14

uniprot entry name :

LOXL2_HUMAN

reactivity :

Human

sequence length :

774

specificity :

This assay has high sensitivity and excellent specificity for detection of human LOXL2. No significant cross-reactivity or interference between human LOXL2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 37.5 pg/ml-2400 pg/ml. Sensitivity: 9.375 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LOXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LOXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505011

ncbi acc num :

NP_002309.1

ncbi gb acc num :

NM_002318.2

uniprot acc num :

Q9Y4K0

ncbi mol weight :

86,725 Da

ncbi summary :

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

uniprot summary :

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated Lys-4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family. Protein type: Secreted; EC 1.4.3.13; Cell adhesion; Oxidoreductase; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: nucleoplasm; extracellular space; membrane; basement membrane; chromosome; nucleus. Molecular Function: protein binding; copper ion binding; electron carrier activity; protein-lysine 6-oxidase activity; chromatin binding; transcription corepressor activity; methylated histone residue binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; collagen fibril organization; transcription, DNA-dependent; protein modification process; protein amino acid deamination; positive regulation of chondrocyte differentiation; response to copper ion; endothelial cell proliferation; histone modification; response to hypoxia; epithelial to mesenchymal transition; endothelial cell migration; sprouting angiogenesis; cell adhesion; negative regulation of transcription, DNA-dependent; aging

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage