catalog number :
MBS904753
products type :
ELISA Kit
products full name :
Mouse interleukin-18 binding prorein, IL-18BP ELISA Kit
products short name :
interleukin-18 binding prorein (IL-18BP)
products name syn :
Mouse interleukin-18 binding prorein (IL-18BP) ELISA Kit; IL18BPa; MC51L-53L-54L homolog gene product; OTTHUMP00000179143; tadekinig-alfa; interleukin-18 binding prorein (IL-18BP)
other names :
interleukin-18-binding protein; Interleukin-18-binding protein; interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein; interleukin 18 binding protein; Interferon gamma-inducing factor-binding protein
products gene name :
IL-18BP
other gene names :
Il18bp; Il18bp; MC54L; Igifbp; IL-18BP; Igifbp; IL-18BP
uniprot entry name :
I18BP_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse IL-18BP. No significant cross-reactivity or interference between mouse IL-18BP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of mouse IL-18BP is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-18BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18BP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18BP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034661.1
ncbi gb acc num :
NM_010531.1
ncbi mol weight :
21,257 Da
uniprot summary :
Function: Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response
size4 :
10x96-Strip-Wells