catalog number :
MBS904711
products type :
ELISA Kit
products full name :
Human Vasohibin-1, VASH1 ELISA Kit
products short name :
vasohibin 1
products name syn :
Human Vasohibin-1 (VASH1) ELISA kit; KIAA1036; ; vasohibin 1
other names :
vasohibin-1; Vasohibin-1; vasohibin-1; vasohibin 1
products gene name :
VASH1
other gene names :
VASH1; VASH1; KIAA1036; KIAA1036; VASH
uniprot entry name :
VASH1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human VASH1. No significant cross-reactivity or interference between human VASH1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 4000 pg/ml. Sensitivity: The minimum detectable dose of human VASH1 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VASH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_055724.1
ncbi gb acc num :
NM_014909.4
ncbi mol weight :
40,957 Da
uniprot summary :
VASH1: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Belongs to the vasohibin family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: extracellular space; endoplasmic reticulum; apical part of cell; cytoplasm. Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to wounding; angiogenesis; negative regulation of blood vessel endothelial cell migration; cell cycle arrest
size4 :
10x96-Strip-Wells