ELISA/assay

Mouse Neutrophil defensin 4, DEFA4 ELISA Kit

MBS904696

48-Strip-Wells

510 USD

ELISA Kit

Mouse Neutrophil defensin 4, DEFA4 ELISA Kit

defensin, alpha 4, corticostatin

Mouse Neutrophil defensin 4 (DEFA4) ELISA kit; DEF4; HNP-4; HP-4; HP4; MGC120099; MGC138296; corticostatin; defensin; alpha 4; defensin; alpha 4; preproprotein; defensin; alpha 4; corticostatin

alpha-defensin 4; Alpha-defensin 4; alpha-defensin 4; alpha-defensin, 28; defensin, alpha, 28; defensin related cryptdin 4; defensin-related cryptdin-4; defensin, alpha, 4; Defensin-related cryptdin-4

DEFA4

Defa4; Defa4; Crp4; Defa28; Defcr4; Defcr4

DEFA4_MOUSE

Mouse

92

This assay has high sensitivity and excellent specificity for detection of mouse DEFA4 . No significant cross-reactivity or interference between mouse DEFA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of mouse DEFA4 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6753626

NP_034169.1

NM_010039.1

P28311

10,272 Da

DEFA4: Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: phospholipid binding. Biological Process: defense response to bacterium; antibacterial humoral response; defense response; membrane disruption in another organism

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800