ELISA/assay

Human Phospholipase A1 member A, PLA1A ELISA Kit

MBS904629

48-Strip-Wells

510 USD

ELISA Kit

Human Phospholipase A1 member A, PLA1A ELISA Kit

phospholipase A1 member A

Human Phospholipase A1 member A (PLA1A) ELISA kit; PS-PLA1; PSPLA1; phosphatidylserine-specific phospholipase A1alpha; phospholipase A1 member A

phospholipase A1 member A isoform 2; Phospholipase A1 member A; phospholipase A1 member A; phosphatidylserine-specific phospholipase A1alpha; phospholipase A1 member A; Phosphatidylserine-specific phospholipase A1; PS-PLA1

PLA1A

PLA1A; PLA1A; PSPLA1; PS-PLA1; NMD; PSPLA1; PS-PLA1

PLA1A_HUMAN

Human

440

This assay has high sensitivity and excellent specificity for detection of human PLA1A. No significant cross-reactivity or interference between human PLA1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20ng/ml. Sensitivity: The minimum detectable dose of human PLA1A is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

332688256

NP_001193889.1

NM_001206960.1

Q53H76

49,715 Da

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.-; Secreted; Phospholipase; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q13.13-q13.2. Cellular Component: acrosomal membrane. Molecular Function: phospholipase A1 activity. Biological Process: phosphatidylserine metabolic process; lipid metabolic process; lipid catabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800