Human Protein-arginine deiminase type-2, PADI2 ELISA Kit | MyBioSource MBS904593 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein-arginine deiminase type-2, PADI2 ELISA Kit

catalog :

MBS904593

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904593

products type :

ELISA Kit

products full name :

Human Protein-arginine deiminase type-2, PADI2 ELISA Kit

products short name :

peptidyl arginine deiminase, type II

products name syn :

Human Protein-arginine deiminase type-2 (PADI2) ELISA kit; RP1-37C10.6; KIAA0994; PAD-H19; PAD2; PDI2; peptidlyarginine deiminase type II; protein arginine deiminase; protein-arginine deiminase type-2; peptidyl arginine deiminase; type II

other names :

protein-arginine deiminase type-2; Protein-arginine deiminase type-2; protein-arginine deiminase type-2; protein-arginine deiminase type II; peptidyl arginine deiminase, type II; PAD-H19; Peptidylarginine deiminase II; Protein-arginine deiminase type II

products gene name :

PADI2

other gene names :

PADI2; PADI2; PAD2; PDI2; PAD-H19; KIAA0994; PDI2

uniprot entry name :

PADI2_HUMAN

reactivity :

Human

sequence length :

665

specificity :

This assay has high sensitivity and excellent specificity for detection of Human PADI2. No significant cross-reactivity or interference between Human PADI2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.68 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PADI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

122939159

ncbi acc num :

NP_031391.2

ncbi gb acc num :

NM_007365.2

uniprot acc num :

Q9Y2J8

ncbi mol weight :

75,564 Da

ncbi pathways :

Protein Citrullination Pathway (142167); Protein Citrullination Pathway (138213)

ncbi summary :

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]

uniprot summary :

PADI2: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family. Protein type: Hydrolase; EC 3.5.3.15. Chromosomal Location of Human Ortholog: 1p36.13. Cellular Component: cytoplasm. Molecular Function: estrogen receptor binding; calcium ion binding; protein-arginine deiminase activity. Biological Process: substantia nigra development; estrogen receptor signaling pathway; chromatin-mediated maintenance of transcription; peptidyl-citrulline biosynthetic process from peptidyl-arginine

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage