Human N-myc proto-oncogene protein, MYCN ELISA Kit | MyBioSource MBS904474 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human N-myc proto-oncogene protein, MYCN ELISA Kit

catalog :

MBS904474

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904474

products type :

ELISA Kit

products full name :

Human N-myc proto-oncogene protein, MYCN ELISA Kit

products short name :

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

products name syn :

Human N-myc proto-oncogene protein (MYCN) ELISA kit; MODED; N-myc; NMYC; ODED; bHLHe37; N-myc proto-oncogene protein; neuroblastoma MYC oncogene; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; oncogene NMYC; pp65/67; v-myc avian; v-myc myelocytomatosis viral related oncogene; neuroblastoma derived (avian)

other names :

N-myc proto-oncogene protein isoform 1; N-myc proto-oncogene protein; N-myc proto-oncogene protein; pp65/67; oncogene NMYC; neuroblastoma MYC oncogene; class E basic helix-loop-helix protein 37; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; Class E basic helix-loop-helix protein 37; bHLHe37

products gene name :

MYCN

other gene names :

MYCN; MYCN; NMYC; ODED; MODED; N-myc; bHLHe37; BHLHE37; NMYC; bHLHe37

uniprot entry name :

MYCN_HUMAN

reactivity :

Human

sequence length :

464

specificity :

This assay has high sensitivity and excellent specificity for detection of human MYCN. No significant cross-reactivity or interference between human MYCN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human MYCN is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYCN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYCN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYCN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

19923312

ncbi acc num :

NP_005369.2

ncbi gb acc num :

NM_005378.5

uniprot acc num :

P04198

ncbi mol weight :

49,561 Da

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

uniprot summary :

N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors. Protein type: Transcription factor; DNA-binding; Oncoprotein. Chromosomal Location of Human Ortholog: 2p24.3. Cellular Component: chromatin; nucleus. Molecular Function: protein dimerization activity; protein binding; DNA binding; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; branching morphogenesis of a tube; negative regulation of astrocyte differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; cartilage condensation; embryonic skeletal morphogenesis; lung development. Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage