Mouse Protein S100-B, S100B ELISA Kit | MyBioSource MBS904472 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Protein S100-B, S100B ELISA Kit

catalog :

MBS904472

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904472

products type :

ELISA Kit

products full name :

Mouse Protein S100-B, S100B ELISA Kit

products short name :

S100 calcium binding protein B

products name syn :

Mouse Protein S100-B (S100B) ELISA kit; NEF; S100; S100beta; OTTHUMP00000174958; S-100 calcium-binding protein; beta chain; S100 beta; S100 calcium binding protein; beta (neural) ; S100 calcium-binding protein; beta; S100 calcium-binding protei; S100 calcium binding protein B

other names :

protein S100-B; Protein S100-B; protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100 protein, beta polypeptide, neural; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

products gene name :

S100B

other gene names :

S100b; S100b; Bpb; AI850290

uniprot entry name :

S100B_MOUSE

reactivity :

Mouse

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse S100B. No significant cross-reactivity or interference between mouse S100B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of mouse S100B is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6677839

ncbi acc num :

NP_033141.1

ncbi gb acc num :

NM_009115.3

uniprot acc num :

P50114

ncbi mol weight :

10,728 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (575140); Cytosolic Sensors Of Pathogen-associated DNA Pathway (575162); DAI Mediated Induction Of Type I IFNs Pathway (575163); Immune System Pathway (575095); Innate Immune System Pathway (575115); MyD88 Cascade Initiated On Plasma Membrane Pathway (575118); MyD88 Dependent Cascade Initiated On Endosome Pathway (575135); MyD88-independent Cascade Initiated On Plasma Membrane Pathway (575142); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (575141); NFkB And MAP Kinases Activation Mediated By TLR4 Signaling Repertoire Pathway (575144)

uniprot summary :

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; metal ion binding; calcium ion binding; calcium-dependent protein binding; receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; cellular calcium ion homeostasis; regulation of cell shape; learning and/or memory; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of cell proliferation; energy reserve metabolic process; positive regulation of synaptic transmission; regulation of cytokine biosynthetic process; memory; regulation of neuronal synaptic plasticity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage