catalog number :
MBS904458
products type :
ELISA Kit
products full name :
Human ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) , RNASE2 ELISA Kit
products short name :
ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
products name syn :
Human ribonuclease; RNase A family; 2 (liver; eosinophil-derived neurotoxin) (RNASE2) ELISA kit; EDN; RNS2; ; ribonuclease; RNase A family; 2 (liver; eosinophil-derived neurotoxin)
other names :
non-secretory ribonuclease; Non-secretory ribonuclease; non-secretory ribonuclease; RNase 2; RNase UpI-2; ribonuclease 2; ribonuclease US; ribonuclease A F3; eosinophil-derived neurotoxin; ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin); Eosinophil-derived neurotoxin; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US
products gene name :
RNASE2
other gene names :
RNASE2; RNASE2; EDN; RAF3; RNS2; EDN; RNS2; RNase 2
uniprot entry name :
RNAS2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human RNASE2. No significant cross-reactivity or interference between human RNASE2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: The minimum detectable dose of human RNASE2 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002925.1
ncbi gb acc num :
NM_002934.2
ncbi mol weight :
18,354 Da
ncbi summary :
The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses. [provided by RefSeq, Oct 2014]
uniprot summary :
RNASE2: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. Belongs to the pancreatic ribonuclease family. Protein type: EC 3.1.27.5; RNA processing; Motility/polarity/chemotaxis; Ribonuclease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: lysosome; extracellular region. Molecular Function: nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity. Biological Process: RNA catabolic process; chemotaxis
size4 :
10x96-Strip-Wells